identification and determination of different alkaloids from atropa belladonna l. by gas chromatography method

background & aim: a. belladonna (family: solanaceae) is one of important pharmaceutical plants which contain tropane ‎alkaloids. tropane alkaloids are distinct group of secondary metabolites of the ‎solanaceae family.  the most important alkaloids of a. belladonna are atropine and hyoscine that are used extensively because of their medicinal properties. therefore identification and ‎determination‏ ‏of different alkaloids rate by gas chromatography (gc) method in iran various areas are necessary. experimental: the accessions were collected from five different regions including ardabil, marzanabad, tonekabon, karaj and ramsar (iran) and all of plant organs were dried. using ‎change of ph‏ ‏and solvents, the alkaloids contents were extracted and the identified and finally ‎the amount of each alkaloid was determined. results & discussion: atropine contents in the accessions of ardebil, marzanabad, tonekabon, karaj, and ramsar were ‎determined 1.8, 1.62, 1.01, 0.76 and 0.67 % respectively. hyoscine contents were determined 0.47, ‎‎0.23, 1.23, 0.16 and 0.09 % respectively. and amount of both total alkaloids were determined 2.66, ‎‎1.85, 2.24, 0.92 and 0.76 % respectively. in this study, the highest amount of atropine (1.8 %), and both ‎total alkaloids which extracted from all dried plant organs belong to the accessions from ardebil and the ‎highest amount of hyoscine (0.46 %) were determined in the accessions from tonekabon. the lowest ‎amount of hyoscine, atropine and both total alkaloids belong to the accessions were from ramsar‎.   industrial and practical recommendations: according to the findings of this study, for extraction more atropine and collection of atropine and   hyoscine from atropa bladonna, the samples of ardabil region can be recommended.